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The six-subunit origin recognition complex (ORC) was originally identified in the yeast Saccharomyces cerevisiae. Yeast ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors, such as Cdc6 and the Mcm proteins. Human homologues of all six ORC subunits have been identified by sequence similarity to their yeast counterparts, but little is known about the biochemical characteristics of human ORC (HsORC).
The origin recognition complex (ORC) is a six-subunit complex that acts as the initiator (the protein that selects the sites for subsequent initiation of replication) at eukaryotic origins of replication.
Bell, SP, et.al. Annu Rev Biochem. 2002;71:333-74.
Thus, assembly of the pre-replicative complex renders chromatin competent for replication. In early G1 phase, the origin recognition complex (ORC) recruits Cdc6, which in turn promotes loading of MCM proteins onto chromatin.
Stoeber,K. et.al. J Cell Sci. 2001 Jun;114(Pt 11):2027-41.
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