Name:Amy L. Altman, Ph.D.
Undergraduate School: Miami University, Oxford, OH,
BA in Microbiology
Graduate Schools: Miami University, Oxford, OH, MS in Microbiology
Vanderbilt University, Nashville, TN, Ph.D. in Molecular Biology
Current Position: Director of Extramural Research, Luminex Corp., Austin, TX
Previous Research Interests:
I am interested in elucidating the mechanisms involved in the
initiation of DNA synthesis is mammalian cells. Using a PCR-based
nascent strand abundance assay, We have determined that a 5.8 kb DNA
fragment containing A 5.8 kb DNA fragment containing a high frequency
initiation region (IR) from the hamster DHFR locus, ori-beta is active
at multiple ectopic chromosomal locations in hamster cells (Altman and
Fanning, Mol. Cell. Biol. 21:1098, 2001). Five non-redundant sequence
elements identified by deletion analysis, including an AT-rich element,
a bent DNA region and a RIP60 binding site, were required for full
initiation activity of ectopic
ori-ï¿½?ï¿½. An AT-rich region
from the human lamin B2 IR substituted for the ori-beta AT-rich element
to restore ori-beta initiation activity whereas replacement with a
similar sized stuffer DNA fragment did not. In the context of ori-beta,
start sites in the lamin B2 AT-rich element were not observed, even
though this element contains the mapped lamin B2 IR. The ability of a
DNA element from a human IR to function in a hamster origin of
replication suggests that a hamster origin might function in human
cells. Indeed, the ectopic 5.8 kb ori-beta DNA fragment was sufficient
to direct initiation in human (Hela and HCT116) cells. Moreover,
mutational analysis confirmed that the sequences in the AT-rich element
were also required for ori-beta activity in the human cell background.
Taken together, the results suggest that specific sequences in the
AT-rich element are required for efficient ori-beta activity and that
elements from different mammalian origins may be functionally
conserved. These results provide evidence of modular sequence elements
required for mammalian DNA replication initiation. Continued study of
this and other mammalian origins should provide additional information
on the composition and architecture of mammalian origins. In addition,
the identified elements are being investigated for potential
protein/DNA interactions at the origin using the chromatin
Name: Bob Ott, Ph.D.
Undergraduate School: Illinois Benedictine College (now known as Benedictine University)
Graduate Schools: Vanderbilt University, Nashville, TN, Ph.D. in Molecular Biology
Current Position: ???
Previous Research Interests:
His first post doc was with Ellen Fanning working on various
aspects of T-Antigen and it's interactions with SV40 replication
His second post doc position was with Dr. George Hill in which he
is studying the effect of inhibitors on a trypanosome alternative
oxidase (TAO). The goal is to identify chemical agents that could be
used to treat trypanosome infections in cattle and humans.
Name: Peijun Yan, Ph.D.
Undergraduate School: Shanghai Second Medical University, China
Graduate Schools: Fudan University, China
Previous Research Interests: I am studying on human DNA helicase
B (HDHB) and Rad52 now. I try to find HDHB function in detail on DNA
repair and find the functional interactions between HDHB and Rad52.
Name: Guoqi Liu, Ph.D.
Current Research Interests:
Undergraduate School: Bryan College, Dayton, TN.
Graduate School: Vanderbilt University
Current Position: Corning Life Science
Name: Xiaorong Zhao, Ph.D.
Undergraduate School: Xiangya School of Medicine, Central South University
Graduate Schools: Xiangya School of Medicine, Central South University
Current Position: LabCorp Laboratory Corporation of America.
Name: Haijiang Zhang, Ph.D.
Undergraduate School: Bejing Normal University
Graduate School: Bejing Normal University
Name: Jeannine Gerhardt, Ph.D.
Graduate School: FSU University Jena, Germany M.S. 1996-2001 Biochemistry
LMU University Munich, Germany
2001-2005 Molecular biology